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Outline

We label a fluorescent probe on a specific region of a biological molecule and bring it back into a cell. We can then visualize how the biological molecule behaves in response to external stimulation. Since fluorescence is a physical phenomenon, we can extract various kinds of information by making full use of its characteristics. For example, the excited energy of a fluorescent molecule donor transfers to an acceptor relative to the distance and orientation between the two fluorophores. This phenomenon can be used to identify interaction between biological molecules or structural change in biological molecules. Besides, we can apply all other characteristics of fluorescence, such as polarization, quenching, photobleaching, photoconversion, and photochromism, in experimentation. Cruising inside cells in a super-micro corps, gliding down in a microtubule like a roller coaster, pushing our ways through a jungle of chromatin while hoisting a flag of nuclear localization signal --- we are reminded to retain a playful and adventurous perspective at all times. What matters is mobilizing all capabilities of science and giving full play to our imagination.

Fields

Medicine, dentistry, and pharmacy, Engineering, Biological Sciences, Biology / Biochemistry

Keywords

Bio-imaging, Fluorescence protein, Chromophore group

Subjects

1. Structure-function relationships of fluorescent protein chromophores
2. Interplay between ambient light and organisms
3. Ultra-fast observation of swimming behavior of micro-organisms

Selected Publications

1. Michikawa, T., Yoshida, T., Kuroki, S., Ishikawa, T., Kakei, S., Kimizuka, R., Saito, A., Yokota, H., Shimizu, A., Itohara, S., and Miyawaki, A.: “Distributed sensory coding by cerebellar complex spikes in units of cortical segments” , Cell Rep.37(6), 109966 (2021).
2. Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki A.: “Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration”, Cell 181(5), 1176-1187 (2020).
3. Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., Tanaka, KZ., Takahashi, M., Ishida, Y., Hata, J., Shimozono, S., Namiki, K., Fukano, T., Kiyama, M., Okano, H., Kizaka-Kondoh, S., McHugh, TJ., Yamamori, T., Hioki, H., Maki, S., Miyawaki, A.: “Single-cell bioluminescence imaging of deep tissue in freely moving animals” , Science 359 (6378), 935-939 (2018).
4. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyawaki, A.: “Genetically encoded tools for optical dissection of the mammalian cell cycle”, Mol. Cell 68, 626-640 (2017).
5. Hama, H., Hioki, H., Namiki, K., Hoshida, T., Kurokawa, H., Ishidate, F., Kaneko, T., Akagi, T., Saito, T., Saido, T., Miyawaki, A.: “ScaleS: an optical clearing palette for biological imaging” , Nat. Neurosci. 18(10), 1518-1529 (2015).

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