Live Cell Super-Resolution Imaging Research Team
Light is a cutting-edge tool for life science research. Development of useful fluorescent probes and the advancement of light microscope technologies have brought us a new world of “live” imaging within a cell. We are developing super-resolution confocal live imaging microscopy (SCLIM) by the combination of a high-speed confocal scanner and a high-sensitivity camera system. With this method we will observe membrane trafficking and organellar dynamics in living cells at high-speed and sub-wavelength space resolution (4D) and elucidate underlining molecular mechanisms. We will also try to extend this technology to medical and pharmacological applications.
Biological Sciences, Complex systems, Interdisciplinary science and engineering, Biology / Biochemistry
membrane traffic, vesicular transport, super-resolution live imaging, confocal microscopy, organelles
- Development of super-resolution live imaging microscopy
- Molecular mechanisms of intracellular membrane trafficking
Yeast trans-Golgi network (red) and clathrin-coated vesicles assembling and leaving there. Captured from a high-speed 3D movie taken by SCLIM2.
- Kurokawa, K., Osakada, H., Kojidani, T., Suda, Y., Asakawa, H., Haraguchi, T., and Nakano, A.:“Visualization of secretory cargo transport within the Golgi apparatus in living yeast cells”, J. Cell Biol. in press.
- Uemura, T., Nakano, R. T., Takagi, J., Wang, Y., Kramer, K., Finkemeier, I., Nakagami, H., Tsuda, K., Ueda, T., Schulze-Lefert, P., and Nakano, A.: “A Golgi-released subpopulation of the trans-Golgi network mediates protein secretion in Arabidopsis”, Plant Physiol. 179:519-532 (2019).
- Ito,Y., Uemura, T., and Nakano, A.:“Golgi Entry Core Compartment functions as the COPII-independent scaffold for ER-Golgi transport in plant cells”, J. Cell Sci. 131:jcs203893 (2018).
- Ito, Y., Toyooka, K., Fujimoto, M., Ueda, T., Uemura, T., and Nakano, A.:“The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells”, Plant Cell Physiol. 58:811-821 (2017).
- Ishii, M., Suda, Y., Kurokawa, K., and Nakano, A.: “COPI is essential for Golgi cisternal maturation and dynamics”, J. Cell Sci. 129:3251-3261 (2016).
|Akihiko Nakano||Team Leader|
|Kazuo Kurokawa||Senior Research Scientist|
|Takuro Tojima||Research Scientist|
|Natsuko Jin||Research Scientist|
|Wataru Yamamoto||Postdoctoral Researcher|
|Daisuke Miyashiro||Special Postdoctoral Researcher|
|Tomohiro Uemura||Visiting Scientist|
|Yasuyuki Suda||Visiting Scientist|
|Yoko Ito||Visiting Scientist|
|Yasuhito Kosugi||Technical Staff Ⅰ|
|Kumiko Ishii||Technical Staff Ⅱ|
|Miho Waga||Technical Staff Ⅱ|