Live Cell Super-Resolution Imaging Research Team

Team Leader

Akihiko Nakano

D.Sci.

Akihiko Nakano

Contact

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nakano [at] riken.jp

Live Cell Super-Resolution Imaging Research Team,
RIKEN Center for Advanced Photonics

#S411 4F Bioscience Building,
2-1 Hirosawa, Wako, Saitama 351-0198 Japan

Related links

Outline

Light is a cutting-edge tool for life science research. Development of useful fluorescent probes and the advancement of light microscope technologies have brought us a new world of “live” imaging within a cell. We are developing super-resolution confocal live imaging microscopy (SCLIM) by the combination of a high-speed confocal scanner and a high-sensitivity camera system. With this method we will observe membrane trafficking and organellar dynamics in living cells at high-speed and sub-wavelength space resolution (4D) and elucidate underlining molecular mechanisms. We will also try to extend this technology to medical and pharmacological applications.

Fields

Biological Sciences, Complex systems, Interdisciplinary science and engineering, Biology / Biochemistry

Keywords

membrane traffic, vesicular transport, super-resolution live imaging, confocal microscopy, organelles

Subjects

  1. Development of super-resolution live imaging microscopy
  2. Molecular mechanisms of intracellular membrane trafficking
SCLIM2 microscope

SCLIM2 microscope

Yeast trans-Golgi network (red) and clathrin-coated vesicles assembling and leaving there. Captured from a high-speed 3D movie taken by SCLIM2.

Yeast trans-Golgi network (red) and clathrin-coated vesicles assembling and leaving there. Captured from a high-speed 3D movie taken by SCLIM2.

Selected Publications

  1. Kurokawa, K., Osakada, H., Kojidani, T., Suda, Y., Asakawa, H., Haraguchi, T., and Nakano, A.:“Visualization of secretory cargo transport within the Golgi apparatus in living yeast cells”, J. Cell Biol. in press.
  2. Uemura, T., Nakano, R. T., Takagi, J., Wang, Y., Kramer, K., Finkemeier, I., Nakagami, H., Tsuda, K., Ueda, T., Schulze-Lefert, P., and Nakano, A.: “A Golgi-released subpopulation of the trans-Golgi network mediates protein secretion in Arabidopsis”, Plant Physiol. 179:519-532 (2019).
  3. Ito,Y., Uemura, T., and Nakano, A.:“Golgi Entry Core Compartment functions as the COPII-independent scaffold for ER-Golgi transport in plant cells”, J. Cell Sci. 131:jcs203893 (2018).
  4. Ito, Y., Toyooka, K., Fujimoto, M., Ueda, T., Uemura, T., and Nakano, A.:“The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells”, Plant Cell Physiol. 58:811-821 (2017).
  5. Ishii, M., Suda, Y., Kurokawa, K., and Nakano, A.: “COPI is essential for Golgi cisternal maturation and dynamics”, J. Cell Sci. 129:3251-3261 (2016).

Members

Akihiko NakanoTeam Leader
Kazuo KurokawaSenior Research Scientist
Takuro TojimaResearch Scientist
Natsuko JinResearch Scientist
Wataru YamamotoPostdoctoral Researcher
Daisuke MiyashiroSpecial Postdoctoral Researcher
Tomohiro UemuraVisiting Scientist
Yasuyuki SudaVisiting Scientist
Yoko ItoVisiting Scientist
Yasuhito KosugiTechnical Staff Ⅰ
Kumiko IshiiTechnical Staff Ⅱ
Miho WagaTechnical Staff Ⅱ
Yutaro ShimizuJRA