Ultrafast Spectroscopy Research Team

Team Leader

Tahei Tahara

D.Sci.

Tahei Tahara

Contact

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tahei [at] riken.jp

Ultrafast Spectroscopy Research Team,
RIKEN Center for Advanced Photonics

2-1 Hirosawa, Wako, Saitama 351-0198 Japan

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Outline

We are developing "femtosecond time-resolved impulsive Raman spectroscopy" (femtosecond is 10-15 second), which enables tracking structural change of the reacting molecule through direct observation of the nuclear motion. Using this method, we successfully elucidated the first event occurring in a photoreceptor protein "Yellow Protein (PYP)" when it stimulates bacteria to escape from the harmful light under light irradiation. We first irradiated PYP with blue light and then shook PYP for observing the nuclear motion moment by moment, like taking "stroboscopic photograph". It was revealed that only 10 trillionths of a second (10-13 seconds) from the irradiation of blue light, specific hydrogen bonds near the light-absorbing site weakened, and then carbon - carbon double bond rotated, generating a peculiar distorted structure. This is a breakthrough achievement that tracks elaborate mechanisms where proteins start to function in real time.

Fields

Chemistry, Physics, Biology / Biochemistry

Keywords

Ultrafast spectroscopy, Nonlinear spectroscopy, Single molecule spectroscopy, Dynamics, Interface

Subjects

  1. Generation of ultrashort pules and development of ultrafast spectroscopic methods
  2. Elucidation and control of molecular dynamics in the condensed phase by ultrafast spectroscopy
  3. Observation and elucidation of molecular dynamics at interfaces by nonlinear spectroscopy
Coherent nuclear motion of Green Fluorescent Protein (GFP)

Coherent nuclear motion of Green Fluorescent Protein (GFP)

Selected Publications

  1. Tahara, S., Takeuchi, S., Abe-Yoshizumi, R, Inoue, K., Ohtani, H., Kandori, H., and Tahara, T.:“Origin of the reactive and non-reactive excited states in the primary reaction of rhodopsins: pH dependence of femtosecond absorption of light-driven sodium ion pump rhodopsin KR2”, J. Phys. Chem. B., 122 , 4784 (2018).
  2. Kuramochi, H., Takeuchi, S., Yonezawa, K., Kamikubo, H., Kataoka M., and Tahara, T.: "Probing the early stages of photoreception in photoactive yellow protein with ultrafast time-domain Raman spectroscopy", Nat. Chem., 9, 660 (2017).
  3. Inoue, K., Singh P. C., Nihonyanagi S., Yamaguchi, S., and Tahara, T.: “Cooperative hydrogen-bond dynamics at a zwitterionic lipid/water interface revealed by 2D HD-VSFG spectroscopy”, J. Phys. Chen. Lett., 8, 5160 (2017).
  4. Singh, P. C., Inoue, K., Nihonyanagi S., Yamaguchi S., and Tahara, T.: “Femtosecond hydrogen-bond dynamics of bulk-like and bound water at positively and negatively charged lipid interfaces revealed by 2D HD-VSFG spectroscopy", Angew. Chem. Int. Ed. 55, 10621 (2016).
  5. Matsuzaki, K., Kusaka, R., Nihonyanagi, S., Yamaguchi, S., Nagata, T., and Tahara, T.: “Partially hydrated electrons at the air/water interface observed by UV-excited time-resolved heterodyne-detected vibrational sum frequency generation spectroscopy”, J. Am. Chem. Soc. 138, 7551 (2016).

Members

Tahei TaharaTeam Leader
Kunihiko IshiiSenior Research Scientist
Satoshi NihonyanagiSenior Research Scientist
Hikaru KuramochiResearch Scientist
Ahmed MohammedPostdoctoral Researcher
Pardeep KumarPostdoctoral Researcher