Advanced Laser Processing Research Team

Team Leader

Koji Sugioka




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ksugioka [at]

Advanced Laser Processing Research Team,
RIKEN Center for Advanced Photonics

2-1 Hirosawa, Wako, Saitama 351-0198 Japan

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The extremely high peak intensity of femtosecond laser allows multiphoton absorption to be localized only at the focal volume in a liquid or a thin film containing protein molecules, where the protein molecules are excited. The excited protein molecules can be then crosslinked to form a solid structure. By scanning the focused laser beam, 3D micro and nanostructures of pure protein can be constructed. Our team took advantages of this technique to create a 3D complex shape of Bovine serum albumin. Furthermore, enhanced green fluorescence protein and enhanced blue fluorescence protein were formed on a single substrate to realize a two-color fluorescence image exhibiting a RIKEN logo. The 3D proteinaceous micro and nanostructures fabricated by this technique will offer many applications including cell culture, tissue engineering, biochips, micromachines, etc.


Engineering, Materials Sciences, Interdisciplinary science and engineering


Femtosecond laser, Laser processing, Micro/nanofabrication, 3D fabrication, Biochip


  1. Development of laser-based 3D micro and nanoprocessing and application for fabrication of micro and nanodevices
  2. Development of high quality, high efficiency, high resolution processing based on beam shaping techniques
  3. Synthesis of nanomaterials by ultafast lasers
  4. Elucidation of laser and matter interactions

(Left) Complex 3D microstructure of Bovine serum albumin (BSA)fabricated by femtosecond laser 3D printing (Right) RIKEN logo fabricated by femtosecond laser 3D printing of EBFP and EGFP.

Selected Publications

  1. Bai S., Serien D., Hu A., and Sugioka K.: “Three-dimensional microfluidic SERS chips fabricated by all-femtosecond-laser-processing for real-time sensing of toxic substances”, Adv. Func. Mater. 28, 1706262 (2018).
  2. He F., Yu J., Tan Y., Chu W., Zhou C., Cheng Y., and Sugioka K.: “Tailoring femtosecond 1.5-μm Bessel beams for manufacturing high-aspect-ratio through-silicon vias”, Sci. Rep. 7, 40785 (2017).
  3. Wu, D., Xu, J., Niu, L., Wu, S., Midorikawa, K., and Sugioka, K.: “In-channel integration of designable microoptical devices using flat scaffold-supported femtosecond-laser microfabrication for coupling-free optofluidic cell counting”, Light Sci. Appl. 4, e228 (2015).
  4. Sugioka K. and Cheng Y.: “Femtosecond laser three-dimensional micro- and nanofabrication”, Appl. Phys. Rev. 1, 041303 (2014).
  5. Sugioka K. and Cheng Y.: “Ultrafast lasers - reliable tools for advanced materials processing”, Light: Sci. Appl. 3, e149 (2014).


Koji SugiokaTeam Leader
Kotaro ObataResearcher
Dongshi ZhangPostdoctoral Researcher
Daniela SerienSpecial Postdoctoral Researcher
Yoshinobu AoyagiVisiting Senior Scientist
Yasutaka HanadaVisiting Scientist
Seisuke NakashimaVisiting Scientist
Felix SimaVisiting Scientist